A1232-21
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
SerumApplications
IHCCrossreactivity
Hu Mo RtShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Alarin (del/E3, Galanin-like Peptide, GALP) (Not for Export EU)
Alarin is a hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2, and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion. Isoform 2 exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2 (By similarity). Cleavage of the signal peptide generates a peptide of 25 amino acids, termed alarin because of the N-terminal alanine and the C-terminal serine. Involved in ganglionic differentiation in neuroblastic tumor tissues.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (formalin-fixed paraffin): 1:500 (DAB) Optimal dilutions to be determined by the researcher.
Positive Control
Mouse/Rat Brain Tissue
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to human Alarin.
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity
Recognizes human Alarin. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
General References:|1. Ohtaki T., Kumano S., Ishibashi Y., Ogi K., Matsui H., Harada M., Kitada C., Kurokawa T., Onda H., Fujino M.; "Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus."; J. Biol. Chem. 274:37041-37045(1999).|2. Kofler B., Moritz K., Fenninger K.; "Differential splicing leads to the novel peptide alarin another member of the galanin-like peptide family."|3. Santic R., Fenninger K., Graf K., Schneider R., Hauser-Kronberger C., Schilling F.H., Kogner P., Ratschek M., Jones N., Sperl W., Kofler B.; "Gangliocytes in neuroblastic tumors express alarin, a novel peptide derived by differential splicing of the galanin-like peptide gene."; J. Mol. Neurosci. 29:145-152(2006).|4. Santic R., Schmidhuber S.M., Lang R., Rauch I., Voglas E., Eberhard N., Bauer J.W., Brain S.D., Kofler B.; "Alarin is a vasoactive peptide."; Proc. Natl. Acad. Sci. U.S.A. 104:10217-10222(2007).USBio References
No references available