Rabbit Anti-Albumin, Bovine Serum (BSA) (HRP)
Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. Anti-Bovine albumin antibody is ideal for investigators involved in serum protein component research.
Applications
Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:500-1:2000 ELISA: 1:5000-1:20,000 Immunohistochemistry: 1:250-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml polyethlene glycol (PEG-8000), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP). Reconstitute with 100ul sterile ddH2O.
Purity
Purified by delipidation, salt fractionation, ion exchange chromatography and dialysis.
Specificity
Recognizes BSA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified bovine serum albumin. Crossreactivity against albumin from other tissues and species may occur.
