Chicken Anti-Albumin, Human Serum (HSA) (MaxLight 490)
MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Albumin is synthesized in the liver and consists of a single polypeptide chain with a molecular weight of 66kD. It is present in all body fluids and is most abundant plasma protein. It acts as a transport protein for long chain fatty acids, prostaglandins, hematin, thyroxin, copper II, cystine and pyridoxal phosphate amongst many other substances that bind more weakly. The average serum concentration of albumin is 42g/L.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Suggested Secondary Antibodies
I1906: IgY, Chicken Pab Rb x Ch I1906-10: IgY, Chicken-HRP Rb x Ch I1906-12: IgY, Chicken-FITC Rb x Ch I1906-14: IgY, Chicken-AP Rb x Ch
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human Serum Albumin
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by affinity chromatography
Specificity
Recognizes human serum and serum albumin Assay by immunoelectrophoresis resulted in a single precipitin arc against albumin in human plasma and purified HSA. There is no crossreactivity to the other human proteins.
