Goat Anti-Annexin A4 (Annexin IV, ANX4, ANXA4, Lipocortin IV, Placental Anticoagulant Protein II, Zymogen Granule Membrane-Associated Protein 36kD, ZAP36)
Annexin A4 is a 321aa, 36kD member of the Annexin protein family. Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin's have a molecular weight of ~35-40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C terminal domain is comprised of four 60-70aa repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth and intracellular signal transduction pathways. Human Annexin A4 shares 92% identity with mouse and rat Annexin A4.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-28K: IgG, H&L (HRP) Pab RbxGt I1904-53L6: IgG, H&L (HRP) Pab RbxGt I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt I1903-88A6: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa1-321 of human Annexin A4 expressed in E. coli (P09525).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous human Annexin A4. Does not crossreact with recombinant human Annexin A1, A2, A3, A5, A6, A7, A8, A9, A10, A11or A13. Species Crossreactivity: Mouse and rat.
