Rabbit Anti-Anterior Gradient 2 (AG-2, AGR2, Secreted Cement Gland Protein XAG-2 Homolog, HPC8)
AG-2 and AG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development(1). Although the roles of AG-2 and AG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-1:1000 Immunohistochemistry (Paraffin): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa55-72, TQTYEEALYKSKTSNKPL, of human Anterior Gradient 2 (Genbank accession no. NP_006399.1) Cellular Localization: Secreted
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 0.2% gelatin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Anterior Gradient 2. Species Sequence Homology: mouse (88%) and bovine (94%)
