A2298-27A1
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
ECrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Goat Anti-a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI) (Glycerol, BSA and Azide Free)
a1Antitrypsin (a1AT), also known as a1Proteinase inhibitor (a1PI), is the most abundant protease inhibitor in blood and a member of the SERPIN family of proteinase inhibitors. Serum levels are typically 1.3mg/ml (2.5uM). a1AT is an acute phase protein and concentrations can rise four-fold during inflammatory episodes or tissue injury. Low levels in circulation have been associated with pulmonary disease such as emphysema. a1AT is a single chain molecule (52kD) that is produced primarily in the liver and to a lesser extent by blood monocytes and intestinal epithelium. Based on association rates, the primary target enzyme for a1AT is believed to be neutrophil elastase. a1AT is a broad-spectrum inhibitor for many serine proteinases. The main role of a1AT in vivo is likely that of a “backup” inhibitor and proteinase scavenger in fluids and tissues. Although the association rates of a1AT with other enzymes are lower, the high concentration in plasma makes it an important inhibitor of activated Protein C, activated F.XI, thrombin and plasmin. Enzyme inhibition by a1AT occurs through proteolytic cleavage between Met358 and Ser359, which induces a conformational change in a1AT locking the enzyme into a stable, inactive 1:1 enzyme-inhibitor complex.
Application
Suitable for use in Immunoelectrophoresis and ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human a1Antitrypsin (a1AT) from plasma.
Form
Supplied as a liquid in PBS. Glycerol free. No preservative added. No stabilizing proteins added.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human alpha1AT as demonstrated by immunoelectrophoresis. A single precipitin arc is observed with normal plasma, of identical mobility to that obtained with purified alpha1AT.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Johnson, D., Travis, J.; Oxidative Inactivation of Human alpha-1-Proteinase Inhibitor; JBC 254: 4022-4026 (1979). 2. Travis, J., Johnson, D.; Human alpha-1-Proteinase Inhibitor; Methods in Enzymology 80: 754-765 (1981). 3. Heeb, M.J., Griffin, J.H.; Physiologic Inhibition of Human Activated Protein C by alpha-1-Trypsin Inhibitor; JBC 263: 1163-11616 (1988). 4. Scott, C.F., Schapira, M., James, H.L,, Cohen, A.B., Colman, R.W.; The Inactivation of Factor XIa by plasma protease inhibitors: Predominant role of alpha-1protease inhibitor and protective effect of high molecular weight kininogen. J. Clin. Invest. 69: 844 (1982).USBio References
No references available