Rabbit Anti-Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10) (Not for Export EU)
APG10 plays a role in autophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule. SUBUNIT: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.
Applications
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immuhistochemistry (IF): 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Cellular Localization
Cytoplasm (Probable).
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to the C-terminal of human APG10 protein
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity
Recognizes human APG10. Species Crossreactivity: human
