A2298-79H4
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG2b,kClone Number
11E19Grade
Affinity PurifiedApplications
WBCrossreactivity
HuAccession #
NP_057041Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-APIP (Probable Methylthioribulose-1-phosphate Dehydratase, MTRu-1-P Dehydratase, APAF1-interacting Protein, CGI-29)
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204aa. An isoform of APIP, APIP2 encodes a 242aa protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa or 293
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full-length his-tagged recombinant protein corresponding to human APIP.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human APIP. Can be used for detection of APIP and APIP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37 2. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13 3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89 4. Cho D-H, Hong Y-M, Lee H-J, Woo H-N, Pyo J-O, Mak TW, Jung Y-K. Induced Inhibition of Ischemic/Hypoxic Injury by APIP, a Novel Apaf-1-interacting protein. J Biol Chem, published on July 15, 2004 as Manuscript M405747200.USBio References
No references available