Technical Data

A2298-88A1
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-APOBEC1 (Apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1, ABEDP, CDAR1, HEPR, apolipoprotein B mRNA editing enzyme)
APOBEC1; apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1; APOBEC-1; BEDP; CDAR1; HEPR; apolipoprotein B mRNA editing enzyme
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:32,000. Western Blot: 1-3ug/ml. Approx. 28kDa band observed in Mouse Spleen lysates (calculated MW of 28.2kDa according to Human NP_001635.2 and 27.5kDa acccording to Mouse NP_112436.1). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See A2298-88A1-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
A synthetic peptide from the C-terminus of aa-DVFYDPRELRKEAC.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human APOBEC1. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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