Technical Data

A2299-36C
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_000473; A8MSL6
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-APOA4, NT (Apolipoprotein A-IV, Apo-AIV, ApoA-IV, Apolipoprotein A4)

The primary translation product of the APOA4 gene is a 396-residue preprotein which after proteolytic processing is secreted into its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human APOA4 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human APOA4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Chien,K.L., Clin. Chim. Acta 388(1-2), 78-83 (2008). 2. Bai,H., Acta Cardiol 63(3), 315-322 (2008).
USBio References
No references available
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