Rabbit Anti-APRIL (A Proliferation Inducing Ligand, CD256, CD256 Antigen, PRO715, Proliferation Inducing Ligand APRIL, TNF and APOL-related Leukocyte Expressed Ligand 2, TALL 2, TALL2, TALL-2, Tumor Necrosis Factor Related Death Ligand, Tumor Necrosis Factor Related Death Ligand 1, TNF-related Death Ligand 1, TRDL 1, TRDL1, TRDL-1, Tumor Necrosis Factor (Ligand) Superfamily Member 13, Tumor Necrosis Factor Ligand Superfamily Member 13, TNFSF13, TNFSF13 Protein, TWE PRIL, TWE-PRIL, UNQ383, UNQ383/PRO715, ZTNF2) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
CD256 is a member of the tumor necrosis factor family, which is expressed in B cells and several tumor cells. The protein is involved in stimulating B and T cell proliferation, triggering humoral immune responses, activating NF-kappaB and inducing cell death. CD256 is thought to play a role in autoimmune diseases and cancer.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-4ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to aa196-210 of human CD256.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD256. Species Crossreactivity: rat and mouse.