Technical Data

A2299-99
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-APRIL, ED2 (a Proliferation Inducing Ligand, TALL-2, TNF and ApoL-related Leukocyte-expressed Ligand 2, TRDL-1a, TNF-related Death Ligand 1a, TNFSF13)

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF-and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-4ug/ml Immunohistochemistry: 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
J9000: Jurkat cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acids within an extracellular domain of human APRIL. Epitope: aa180-230
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human APRIL. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hahne, M., Kataoka, T., Schroter, M., et al., APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J. Exp. Med.188(6: 1185-90 (1998). 2. Shu, H.B., Hu, W.H., Johnson, H.. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J. Leukoc. Biol. 65(5): 680-3 (1999). 3. Kelly, K., Manos, E., Jensen, G., et al., APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 60(4): 1021-7 (2000). 4. Yu, G., Boone, T., Delaney, J., et al., APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat. Immunol. 1(3): 252-6 (2000). 5. Wu, Y., Bressette, D., Carrell, J.A., et al., Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS. J. Biol. Chem. 275(45): 35478-85 (2000). 6. Marsters, S.A., Yan, M., Pitti, R.M., et al., Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr. Biol. 10(13): 785-8 (2000). 7. Rennert, P., Schneider, P., Cachero, T.G., et al., A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth. J. Exp. Med. 192(11): 1677-84 (2000). 8. Gross, J.A., Johnston J., Mudri, S., et al., TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease. Nature 404: 995-9 (2000). 9. Ware, C.F., APRIL and BAFF connect autoimmunity and cancer. J. Exp. Med. 192(11): F35-8 (2000).
USBio References
No references available
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