Technical Data

A3570-70E
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Hu Mo
Accession #
NP_005735.2
Gene ID
25820, 23806
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-ARIH1 (E3 Ubiquitin-protein Ligase ARIH1, H7-AP2, HHARI, Monocyte Protein 6, MOP-6, Protein Ariadne-1 Homolog, ARI-1, UbcH7-binding Protein, UbcM4-interacting Protein, Ubiquitin-conjugating Enzyme E2-binding Protein 1, ARI, MOP6, UBCH7BP, HUSSY-27)

E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 0.2-1ug/ml, U937 lysate observed on ~65kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-HEGYEKDLWEYIED, from human ARIH1, at C-terminal (NP_005735.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ARIH1. Species sequence homology: Bovine and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. LaVoie MJ, Cortese GP, Ostaszewski BL, Schlossmacher MG. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem. 2007 Dec;103(6):2354-68. 2. Wong ES, Tan JM, Wang C, Zhang Z, Tay SP, Zaiden N, Ko HS, Dawson VL, Dawson TM, Lim KL. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem. 2007 Apr 20;282(16):12310-8.
USBio References
No references available
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