Goat Anti-ARPC4 (Actin related protein 2/3 complex, subunit 4, 20kD, ARC20, MGC13544, p20-Arc, Arp2/3 protein complex subunit p20)
ARPC4; actin related protein 2/3 complex, subunit 4, 20kD; ARC20; MGC13544; p20-Arc; Arp2/3 protein complex subunit p20; actin related protein 2/3 complex subunit 4; actin related protein 2/3 complex, subunit 4 (20 kD)
ARPC4 (actin-related protein 2/3 complex subunit 4), is an actin-binding element of the evolutionary conserved Arp2/3 protein complex, implicated as a regulator of actin polymerization, and mediator of branched actin networks.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:64,000. Western Blot: 0.01-0.03ug/ml. Approx. 19kDa band observed in Human Lymph Node lysates (calculated MW of 19.7kDa according to NP_005709.1). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See A3576-03-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
A synthetic peptide from the C-terminus of aa-ERHNKPEVEVR.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human ARPC4. Species Crossreactivity: mouse.
