Goat Anti-Arrestin 1, beta (ARRB1, ARB1, ARR1)
The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (b-Arrestin 1), Arrestin 3 (b-Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, b-Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, b-Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38a, and JNK3. These b-Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 3-10ug/ml with the appropriate secondary reagents to detect b-Arrestin 1 in paraffin-embedded sections of normal human spleen and human colon cancer. Optimal dilution determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived full-length recombinant human b-Arrestin 1, Met1-Arg418.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human b-Arrestin 1.
