Mouse Anti-Arrestin 1, beta (ARRB1, ARB1, ARR1) (MaxLight 490)
MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
b-arrestin 1 (ARRB1, ARB1, ARR1) is a member of the arrestin protein family, which also includes visual arrestin, cone arrestin and b-arrestin 2. b-arrestins bind phosphorylated heptahelical G protein coupled receptors to desensitize and target them to clathrin-coated pits for endocytosis. b-arrestin 1 acts as a cofactor in the b-adrenergic receptor kinase (BARK)-mediated desensitization of b-adrenergic receptors, resulting in specific dampening of cellular responses to stimuli such as hormones, neurotransmitters or sensory signals (1). b-arrestin 1 exists as two alternately spliced transcripts, encoding two isoforms. Isoform A is a longer variant, while isoform B lacks an alternate in-frame exon compared to isoform A, resulting in a shorter protein (1). The functions of the isoforms have not been fully described. b-arrestin 1 is a cytosolic protein, and is widely expressed in cells, particularly in the central nervous system and in peripheral blood leukocytes (2).
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilutions
FLISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Controls
Mouse RAW264.7 nuclear extracts, rat PC-12 cell lysates.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the C-terminal region of human beta-arrestin 1, which shares 84% sequence homology with mouse and rat.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography.
Specificity
Reactivity has been confirmed with mouse RAW264.7nuclear extracts and rat PC-12 cell lysates. Based on amino acid sequence homology, reactivity with human is expected.
