Technical Data

A3619-80C
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_004307
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ASCL1 (L68) (Class A Basic Helix-loop-helix Protein 46, Achaete-scute Homolog 1, ASH-1, hASH1, bHLHa46, ASH1, BHLHA46, HASH1)

Mash1 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. This protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in the gene encoding Mash1 may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding L68 of human ASCL1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ASCL1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Osada,H., Cancer Res. 68(6), 1647-1655 (2008). 2. Gregory,G.D., Mol. Cell. Biol. 27(24), 8466-8479 (2007). 3. Wang,X.Y., Lab. Invest. 87(6), 527-539 (2007).
USBio References
No references available
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