Rabbit Anti-ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, MCPH5)
ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.
Molecular Weight
218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol. 14, No. 15 2155-2165.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: Tested in direct ELISA against the ASPM immunogen. Immunohistochemistry (Paraffin): 1:300. Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm. Optimal dilutions to be determined by the researcher.
Positive Control
Human ductal carcinoma tissue
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Linear peptide corresponding to human ASPM conjugated to KLH. (NP_060606). Epitope: Internal. Species Sequence Homology: chimpanzee and primate
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human ASPM. Species Crossreactivity: rat
