Technical Data

A4000-91C
Clone Type
Monoclonal
Host
Mouse
Source
Rabbit
Isotype
IgG1
Clone Number
6D138 (VE121G9)
Grade
Ascites
Applications
IHC WB
Crossreactivity
Ca Hu Mo Rb
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-ATPase 1, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA1)
Applications
Suitable for use in Immunohistochemistry, Western Blot and Functional Applications. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:500 Western Blot: 1:2500, detects a band of ~110kD Functional Applications: inhibits the crystallization of SERCA1 ATPase induced by vanadate Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified rabbit skeletal muscle sarcoplasmic reticulum.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Ascites
Specificity
Recognizes rabbit SERCA1 ATPase (sarcoplasmic/endoplasmic reticulum calcium ATPase 1) in human, mouse, canine and rabbit type II (fast) skeletal muscle. Detects a band of ~110kD by Western Blot.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
A monoclonal antibody to the Ca2+ ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical study: A.O. Jorgensen, et al.; Cell Motil. Cytoskeleton 9, 164 (1988)
USBio References
No references available
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