Rabbit Anti-Aurora B (STK12, Serine Threonine Kinase 12, Aurora Kinase B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2) (PE)
Human aurora A (aurora related kinase-1 (ARK-1), Aik1, STK15, BTAK, aurora2), and human aurora B (aurora related kinase-2 (ARK-2), Aik2, STK12, aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis.1, 2 Aurora B has unique genetic loci relative to Aurora A suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.1, 3 Aurora B exists in a complex with survivin and inner centromere protein (INCENP) in mitosis, and its kinase activity is stimulated by survivin binding and cell cycle-dependent phosphorylation.4 Phosphorylation of INCENP by aurora B stimulates aurora B kinase activity .5
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1-3ug/m Western Blotting: 1-3ug/ml Immunoprecipitation: 7ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the N-terminal region of the human Aurora B protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Purity
Epitope-affinity purified from rabbit antiserum.
Specificity
Reacts with the human Aurora B. On Western blots, it identifies the target band at ~39kD. A band of unknown origin is also observed at ~ 75kD. Reactivity has been confirmed with human HeLa cervical carcinoma cell lysates.
