Technical Data

A5801-01F
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Bo Ca Hu Mo Po Rt
Accession #
NP_000027.1
Gene ID
270
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-AMPD1 (AMP Deaminase 1, AMP Deaminase Isoform M, Myoadenylate Deaminase)

AMP deaminase (AMPD) is an allosteric enzyme involved in the regulation of adenosine metabolism. It catalyzes a central reaction in purine nucleotide biosynthesis where AMP is deaminated to IMP liberating ammonia. There are three functional isoforms of AMPD. AMPD1 (E.C. No 3.5.4.6.) is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. This protein is the predominant member of AMPD multi-gene family and is considered as the sensor of the cell's changing energy requirements. AMPD1 deficiency causes irregular muscle metabolism due to lower rate of ATP degradation, phosphocreatine hydrolysis and accumulation of lactic acid. Mutated AMPD1 expression has been detected in neuromuscular disorders, exercise-induced skeletal muscle myopathies and congestive heart failures due to coronary artery diseases.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-HMFSSKSPKPQE, from AMPD1, at the internal region of the protein (NP_000027.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes AMPD1. Species sequence homology: Bovine, canine, human, mouse, porcine and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hand BD, Roth SM, Roltsch MH, Park JJ, Kostek MC, Ferrell RE, Brown MD. AMPD1 gene polymorphism and the vasodilatory response to ischemia. Life Sci. 2006 Sep 5;79(15):1413-8. Epub 2006 May 16.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net