Rabbit Anti-BACE, CT (Beta-Secretase 1, Aspartyl Protease 2, ASP2) [KO Validated]
Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
Applications
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml Immunohistochemistry (paraffin): 2.5ug/ml Immunocytochemistry: 10ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Positive Controls
Human brain tissue lysate, 3T3/NIH cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. Epitope: within the last 50aa. Species Sequence Homology: guinea pig: (94%), rat: (94%) and bovine: (94%)
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human BACE. Species Crossreactivity: mouse
