B0003-51E
Clone Type
MonoclonalHost
RabbitSource
HumanIsotype
IgGClone Number
9j108(D24A9)Grade
PurifiedApplications
WBCrossreactivity
Hu Mk Mo RtGene ID
572Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Bad (Bcl2 Antagonist of Cell Death)
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival 3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues surrounding Pro102 of human Bad. Species sequence homology: bovine (100%)
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of total human Bad. Does not cross-react with related proteins. Species crossreactivity: mouse, monkey and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Yang, E. et al. (1995) Cell 80, 285–291. 2. Zha, J. et al. (1996) Cell 87, 619–628. 3. Datta, S.R. et al. (1997) Cell 91, 231–241. 4. Peso, L. et al. (1997) Science 278, 687–6895) 5. Bonni, A. et al. (1999) Science 286, 1358–1362. 6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859–34867. 7. Harada, H. et al. (1999) Mol. Cell 3, 413–422. 8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865–25869. 9. Lizcano, J. et al. (2000) Biochem. J. 349, 547–557. 10. Datta, S. et al. (2000) Mol. Cell 6, 41–51.USBio References
No references available