MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
CD268 is a member of the tumor necrosis factor receptor family, which is expressed by B cells. CD268 plays a role in BAFF induced B cell development and survival, and is thought to be involved in B cell autoimmune diseases
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 2-5ug/ml. Detects a band of ~20kD in human, mouse and rat spleen lysates. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1903-30T: IgG, H&L, X-adsorbed (Biotin) Pab Sh xRb I1903-30H: IgG, H&L, X-adsorbed (FITC) Pab Sh xRb I1903-30J: IgG, H&L, X-adsorbed (FITC) Pab Sh xRb I1903-30U: IgG, H&L, X-adsorbed (HRP) Pab Sh xRb
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a region from the C-terminus of the human BAFF Receptor.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human BAFF Receptor. Species Crossreactivity: rat and mouse.