Technical Data

B0003-84C
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Purified
Applications
IHC IP WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-BAG 1, CT (Bcl2 Interacting Protein 1, HAP1, RAP46, Bcl-2 associated athanogene-1)

BAG1 binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.

Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 1:2500 Immunoprecipitation: Used with transfected cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa204-219, C204QETERLQSTNLALAE219 of mouse BAG-1.
Form
Supplied as a liquid in PBS, 0.05% sodium azide
Purity
Purified
Specificity
Recognizes mouse BAG-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity: S. Takayama, et al.; Cell 80, 279 (1995). 2. Interleukin-2 (IL-2) upregulates BAG-1 gene expression through serine-rich region within IL-2 receptor beta c chain: M. Adachi, et al.; Blood 88, 4118 (1996). 3. Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1: H.G. Wang, et al.; PNAS 93, 7063 (1996). 4. Role of Bag-1 in the survival and proliferation of the cytokine-dependent lymphocyte lines, Ba/F3 and Nb2: C.V. Clevenger, et al.; Mol. Endocrinol. 11, 608 (1997). 5.Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002)
USBio References
No references available
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