B0055-01A
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IF IHC IP WBCrossreactivity
Hm Hu Mk PoAccession #
NP_001179Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Bak (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer)
Bak is a 23kD homolog of the apoptosis inhibitor, Bcl-2 with an apparent molecular weight of 30kD on SDS-PAGE. Bak is a member of the Bcl-2 family of proteins and contains the bcl-2 homology regions (BH-) 1, 2 and 3. Unlike Bcl-2, Bak promotes apoptosis by interacting with and counteracting the anti-cell death effect of Bcl-2. Enforced expression of Bak induces rapid and extensive apoptosis of serum-deprived fibroblasts (1). However, Bak can inhibit cell death in an Epstein-Barr virus transformed cell line (2).
Applications
Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot (ECL): 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher.
Positive Controls
HeLa Heat Shocked Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa2-14, ASGQGPGPPRQEC, of human Bak conjugated to KLH.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes human Bak at ~30kD. Species Crossreactivity: hamster, monkey and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Chittenden, T., Harrington, E.A., OíConnor, R., Flemington, C., Lutz, R.J., Evan, G.I. and Guild, B.C. Nature 374: 733–736(1995). 2. Kiefer, M.C. (1995) Nature 374: 736–739. 3. Wang, G-Q. et al (2001) J. Biol. Chem. 276(36): 34307–34317.USBio References
No references available