Technical Data

B0055-10D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001179
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Bak, BH3 Domain (Bcl-2 Homologous Antagonist/Killer, Apoptosis Regulator BAK, Bcl-2-like Protein 7, Bcl2-L-7, BAK1, BCL2L7, CDN1)

BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:2000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 56-91 of human Bak
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human Bak

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003). 2. Mikhailov, V., et al., J. Biol. Chem. 278(7):5367-5376 (2003). 3. Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002). 4. Bellosillo, B., et al., Blood 100(5):1810-1816 (2002). 5. Grutkoski, P.S., et al., Shock 17(1):47-54 (2002).
USBio References
No references available
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