Rabbit Anti-BAP29 (B Cell Receptor-Associated Protein 29, BCR-Associated Protein 29)
Description
Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family (1,2). These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors (3). Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins (3). Bap29 possesses multiple isoforms.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug’ml Optimal dilutions to be determined by the researcher.
Positive Control
Human heart tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide BAP29 17aa near the center of human Bap29 |(Genbank accession No. NP_001008405).
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.|
Purity
Purified by immunoaffinity chromatography.|
Specificity
Recognizes human BAP29. Species Crossreactivity: mouse and rat.
