Technical Data

B0130-26
Clone Type
Polyclonal
Host
Guinea pig
Source
Human
Isotype
IgG
Grade
Serum
Applications
IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Guinea pig Anti-Basic Hair Keratin, K84 (KRT84, Keratin 84, HB4, Hb-4, K84, Keratin, Type II Cuticular Hb4, Keratin-84, KRTHB4, Type II Hair Keratin Hb4) (Not for Export EU)

Type II hair keratin is one of the two types of hair keratin. It is a basic protein which heterodimerizes with type I hair keratins to form hair and nails.

Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen): 1:200. Incubate at RT for 1 hour. Western Blot (ECL): 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human basic hair (trichocytic) keratin K84, conjugated to KLH. (Formerly designated hHb4; IAA VGS RPI HCG VRF).
Form
Supplied as a liquid in 0.5% BSA, 0.09% sodium azide.
Purity
Serum
Specificity
Recognizes human hair keratin K84 expressed starting from the upper layers of the filiform papillae of tongue epithelium and in mouse tail buds. No staining of anagen hair follicle. |Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Langbein, L., et al., J. Biol. Chem. 276: 35,123-35,132 (2001). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).
USBio References
No references available
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