Technical Data

B0140-01A
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG2a,k
Clone Number
8J289 (SAP7F407)
Grade
Affinity Purified
Applications
IC IF IHC IP WB
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Bassoon

Bassoon is a 420kD protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry/Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:400 Immunohistochemistry (Paraffin): 1:10-1:2000 Immunoprecipitation: 2.5ug Western Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to rat Bassoon
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat bassoon at ~416kD. Additional bands between 97kD and 400kD have been identified and are reported to be proteolytic degradation products of bassoon. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Tom Dieck, S., Sanmarti-Vila, L., Langnaese, K., Richter, K, Kindler, S., Soyke, A., Wex, H, Smalla, K-H., Kampf, U., Franzer, J-T., Stumm, M., Garner, C.C., Gundelfinger, E.D. (1998) J. Cell Biol. 142: 499-509. 2. Richter, K., Langnaese, K., Kreutz, M.R., Olias, G., Zhai, R., Scheich, H., Garner, C.C., Gundelfinger, E.D. (1999) J. Comp. Neurol. 408: 437-448. 3. Winter, C., tom Dieck, S., Boeckers, T.M., Bockmann, J., Kanmpf, U., Sanmarti-Vila, L., Langnaese, K., Altrock, W., Stumm, M., Soyke, A., Wieacker, P., Garner, C.C., Gundelfinger, E.D. (1999) Genomics 57: 389-397. 4. Cases-Langhoff, C., Voss, B, Garner, A.M., Appeltauer, U., Takei, K., Kindler, S., Veh, R.W., De Camilli, P., Gundelfinger, E.D., Garner, C.C. (1996) Eur. J. Cell Biol 69: 214-223. 5. Phillips, G.R., et al. (2001) Neuron 32: 63-77. 6. Morales, M., Colicos, M.A., Goda, Y. (2000) Neuron 27: 539-550. 7. Ohtsuka, T.; Takao-Rikitsu, E.; Inoue, E.; Inoue, M.; Takeuchi, M.; Matsubara, K.; Deguchi-Tawarada, M., Satoh, K., Morimoto, K., Nakanishi, H., Takai, Y. (2002) J Cell Biol 158(3): 577-590. 8. Shiraishi, Y., Mizutani, A., Mikoshiba, K., Furuichi, T. (2003) Mol Cell Neurosci 22(2): 188-201. 9. Lee, H.K., Takamiya, K., Han, J.S., Man, H., Kim, C.H., Rumbaugh, G., Yu, S., Ding, L., He, C., Petralia, R.S., Wenthold, R.J., Gallagher, M., Huganir, R.L. (2003) Cell 112(5):631-643. 10. Hirbec, H., Francis, J.C., Lauri, S.E., Braithwaite, S.P., Coussen, F., Mulle, C., Dev, K.K., Couthino, V., Meyer, G., Isaac, J.T.R., Collingridge, G.L., Henley, J.M. (2003) Neuron 37(4): 625-638.
USBio References
No references available
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