Mouse Anti-Bcl-2 (B Cell Leukemia 2) (PE)
The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells.
Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 Western Blot: Unconjugated Optimal dilutions to be determined by the researcher..
Flow Cytometry Protocol
Incubations and washes using a buffer containing 0.5% Tween-20 to permeabolize cell, were done at RT. 1. Cultured Nalm-6 human tumor cells were fixed for 20 min with 0.5% freshly prepared buffered formaldehyde after which they were washed two times. 2. Five x 10e5 cells per tube were incubated 45 minutes with 80ul of B0807-06H at a 1:50 dilution. 3. Cells were then washed three times, fixed and analyzed using a BD FACstar plus. 4. Cells stained positive with a mean shift of 0.78 log 10 fluorescent units when compared to a tube where cells were pre blocked with 20ul of 0.5mg/ml B0807-06E.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of enzymatic activity
Immunogen
Human bcl-2 peptide 41-54.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the intracellular 26kD of the human Bcl-2 protein.
