Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipiation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.

Immunoprecipitation (Use Protein A): 2ug/mg protein lysate Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20min followed by cooling at RT for 20min. Optimal dilutions to be determined by the researcher.

Hodgkin’s lymphoma

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide corresponding to residues 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. Cellular Localization: Cytoplasmic and cell/nuclear membrane.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

Purified by Protein A affinity chromatography.

Recognizes Bcl-X at 27kD. Does not react with Bcl-2a or Bax protein. Species Crossreactivity: Human, mouse, rat and porcine.