Rabbit Anti-BD1 (beta Defensin-1) (HRP)
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfide and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Control Peptide
B0900-09A BD1, Control Peptide (beta Defensin-1)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Note: Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide corresponding to 16aa near the N-terminus of mature mouse MBD-1, conjugated KLH. Species Sequence Homology: rat, 87%. No significant homology exists with human beta-defensin 1 or other defensins.
Form
Supplied as a liquid in PBS, pH 7.4. Labeled with horseradish peroxidase (HRP). No preservative added. No stabilizing proteins added.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse BD-1.
