B0901-03B-ML490
Clone Type
PolyclonalHost
GoatSource
HumanConjugate
MaxLight™490Isotype
IgGGrade
Affinity PurifiedApplications
FLISA WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Goat Anti-BD2 (beta Defensin-2, beta 2 Defensin, Defensin beta 2, BD 2, BD-2, DEFB2, DEFB-2, HBD 2, HBD2, HBD-2, DEFB102, Skin Antimicrobial Peptide 1, SAP1) (MaxLight 490)
Beta 4 Defensin, Defensin 4 beta, Defensin beta 4, DEFB4, DEFB-4
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-terminal active portion of the protein.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human Defensin beta 2.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Defensin beta-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Bals, R. et al. (1998) Human beta-Defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J. Clin. Invest. 102: 874-880. 2. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721. 3. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721.USBio References
No references available