Rabbit Anti-Beclin-1 (Coiled-coil Myosin-like Bcl-2-Interacting Protein)
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA, Immunocytochemistry/Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 2.5ug/ml. Require antigen retrieval by heat mediation with a citrate buffer (pH6). Immunocytochemistry/Immunofluorescence: 10-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 17 amino acids near the center of human Beclin-1. Epitope: aa40-90. Species Sequence Homology: bovine and porcine
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Beclin-1. Species Crossreactivity: mouse and rat.
