This product has been optimized to detect biotinylated protein markers (P9106-75) by Western Blot. Other applications not tested.

Western Blot: 1:1000-1:3000 Note: To detect other biotinylated proteins by Western Blot: 1:100 in PBS, 5% BSA, 0.05% Tween 20 with overnight incubation. Optimal dilutions to be determined by researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

P9106-75: Prestained Protein Marker, Broad Range P9106-25: Protein MW Marker, Detection (Biotin)

Biotin

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Purified by affinity chromatography.

Recognizes biotin.