Fusion Partners: Spleen cells from immunized Balb/c were fused with cells of a myeloma cell line.

Recommended Negative Controls: Mouse IgG1 Negative Control

Immunoprecipitation Haemagglutination Optimal working dilutions to be determined by researcher.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Human erythrocytes.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes the kell blood group antigen Kpbc. The kell blood group antigens are carried on a 93kD erythrocyte membrane glycoprotein. In addition clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but does not react with Ko erythrocytes.