Rabbit Anti-Bone Morphogenic Protein Receptor 1A (CD292, BMPR-1A, Bone Morphogenetic Protein Receptor Type IA, Serine Threonine Protein Kinase Receptor R5, SKR5, Activin Receptor-like Kinase 3, ALK-3, ACVRLK3) (MaxLight 650)
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Bone Morphogenetic Proteins (BMPs) are members of the TGF-β superfamily of cytokines that affect bone and cartilage formation1. Mature BMPs are 30-38kD proteins that assume a TGF beta-like cysteine knot configuration1. Most BMPs are homodimers, but bioactive natural heterodimers have also been reported1. BMPs are involved in embryogenesis and morphogenesis of various tissues and organs. They create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. BMPs also regulate the growth, differentiation, chemotaxis, proliferation, and apoptosis of mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells, and are responsible for normal dorsal/ventral patterning. Two type I receptors have been characterized, BMPR-IA (also designated activin receptor-like kinase ALK-3, BRK-1 and SKR5), and BMPR-IB (also designated ALK-6 and SKR 6), that bind to bone morphogenetic proteins (BMP)-2, BMP-4, and osteogenic protein (OP)-1 (also designated BMP-7)2-5. Type I receptors involved in BMP signaling can independently bind the various BMP family proteins in the absence of type II receptors. BMPR-IA and BMPR-IB are thought to mediate distinct effects on gene expression, cell differentiation, and morphogenesis in a dose dependent fashion 2,6.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from an internal region of the human BMPR-1A (bone morphogenetic protein receptor type 1A, Serine/threonine protein kinase receptor R5 (SKR5), activin receptor-like kinase-3 (ALK-3)), which differs from mouse/rat BMPR-1A by one nonconservative amino acid replacement.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human BMPR-1A at ~60kD. Reactivity has been confirmed with human SK-OV-3, NGP96, SKMEL-19, SK-MEL-37, A375, MeWo cell lysates and mouse ovary homogenates. Species Crossreactivity: rat and mouse
