Rabbit Anti-Borrelia burgdorferi (Lyme Disease) (Biotin)
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
Applications
Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed/paraffin embedded sections Western Blot: >1:1,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Whole cell preparation from B. burgdorferi.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added. Labeled with Biotin.
Specificity
Recognizes Borrelia burgdorferi (Lyme Disease) at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Crossreacts with Treponema pallidum, B. hermsii, B. parkerii.
