Rabbit Anti-Brain Derived Neurotrophic Factor, pro (proBDNF)
Abrineurin
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-5ug/ml. Detects a 35kD band corresponding to the molecular weight of proBDNF. Immunohistochemistry: 1-5ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide (C-ELLDEDQKVRPNEE) corresponding to aa 69-82 of human BDNF precursor protein
Form
Supplied as a lyophilized powder. Reconstitute in 20ul of sterile water.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human proBDNF. Species Crossreactivity: mouse and rat. Expected to recognize other mammalian proBDNF.
