B2702-01D-ML490
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™490Isotype
IgG1Clone Number
10B302 (18H5)Grade
Affinity PurifiedApplications
FLISA WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Brain Natriuretic Peptide, Pro, NT, aa 13-27 (NT-proBNP, N-terminal prohormone brain natriuretic peptide) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
NT-proBNP is a cleavage product during the proteolytic activation of BNP. NT-proBNP is a secreted protein which is a member of the natriuretic peptide family, which is predominantly secreted from the cardiac ventricles in response to volume and pressure overload. NT-proBNP is more stable than BNP in serum, and has become a useful biomarker of heart failure.
Applications
Suitable for use in FLISA and Western Blot. Other applications have not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
aa13-27 from N-terminal of Pro Brain Natriuretic Peptide
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the amino-terminal fragment at aa13-20 of human pro-brain natriuretic peptide at ~9kD.