Mouse Anti-Brain-Specific Angiogenesis Inhibitor 3 (BAI3)
Human BAI-3 (brain-specific angiogenesis inhibitor 3) is a 177kD, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI-3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296-508), and one GPS domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI-3 is 98% aa identical to mouse BAI-3.
Applications
Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: Direct Western Blot: 1ug/ml using Recombinant Human BAI3 under non-reducing conditions only. Flow Cytometry: 0.25ug/10e6 cells using HEK293 Human Cell Line Transfected with Human BAI3 and eGFP Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ala25-Thr880 from human Brain-Specific Angiogenesis Inhibitor 3, expressed in NS0 cells. Uniprot/Accession Number: O60242
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Brain-Specific Angiogenesis Inhibitor 3.
