Technical Data

C0010-12B
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1
Clone Number
13A17 (60.11)
Grade
Affinity Purified
Applications
FC FLISA IHC IP WB
Crossreactivity
Hm Hu Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-C1q Receptor, gamma (gC1qR, C1qBP, Complement Component 1, Q Subcomponent-binding Protein) (FITC)

This monoclonal antibody recognizes a cell membrane C1q binding molecule that recognizes the globular heads of C1q. It is also present in plasma and the extracellular matrix. The molecule is an unusually acidic, single chain protein with an apparent molecular weight of 33kD. It does not possess a conventional sequence motif compatible with a membrane spanning segment nor a consensus site for a GPI anchor. gC1q-R migrates as a single chain under both reducing and non-reducing conditions, but it behaves as an oligomer on gel-filtration in non-dissociating conditions. Its multimer formation may be a critical process by which the gC1q-R molecule increases its affinity for multivalent ligands such as C1q. gC1q-R has been shown to inhibit complement activation by preventing the binding of C1q to antibodies, suggesting that the binding site for gC1q-R and the binding site for immune complexes, which are present on the C1q globular 'heads', may be located at the same position. gC1q-R is capable of interacting with several proteins involved in blood clotting, namely, thrombin, prothrombin, the heparinbinding form of vitronectin, the ternary complex, vitronectin-thrombin- antithrombin, as well as high-molecular-weight kininogen and Hageman factor. Besides its role in the complement pathway, gC1q-R participates in enhancement of Fc-receptor and CR1-mediated phagocytosis, procoagulant activity on platelets, and chemotactic activity on mast cells, eosinophils, neutrophils, and fibroblasts. gC1q-R is expressed on a wide variety of cells and can serve as a binding site for plasma and microbial proteins. Its antigenic sites may be cryptic on cells in suspension but become exposed when the cells are fixed by bifunctional cross-linkers. Probably it is also expressed on the cell membrane as a tetramer. Crosslinking or activation may thus bring about a tetrameric assembly of gC1q-R followed by a conformational change within the molecule, thereby exposing epitopes which are otherwise hidden. A form of GC1q-R is also found inside the cell. Intracellular gC1q-R has been shown to bind the cytoplasmic tail of the a1B-adrenergic receptor and to PKCu.

Applications
Suitable for use in Western Blot, Flow Cytometry, Functional Studies, FLISA, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50 Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to aa74-282 from mature GC1q-R.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes epitopes situated within the NH2-terminal stretch of gC1q-R corresponding to aa76-93. This clone recognizes the putative C1q binding site and reacts with the mature form, but has poor or no reactivity with the truncated form, lacking residues 74-95. Species Crossreactivity: rat and Syrian hamster.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ghebrehiwet, B et al; Identification of functional domains on gC1Q-R, a cell surface protein that binds to the globular "heads" of C1Q, using monoclonal antibodies and synthetic peptides. Hybridoma 1996, 5: 333. 2. Ghebrehiwet, B et al; Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate to form a complex. J Immunol 1997, 159: 1429. 3. Ghebrehiwet, B et al; gC1q-R/p33, a member of a new class of multifunctional and multicompartmental cellular proteins, is involved in inflammation and infection. Immunol Rev 2001, 180: 65. 4. Grace, K et al; Surface expression of complement receptor gC1q-R/p33 is increased on the plasma membrane of human spermatozoa after capacitation. Biol Reprod 2002, 66: 823. 5. Peerschke, E et al; gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis. Infect Immun 2006, 74: 4418. 6. Sansonno, D et al; Role of the receptor for the globular domain of C1q protein in the pathogenesis of hepatitis C virau-related cryoglobulin vascular damage. J Immunol 2009, 183: 6013.
USBio References
No references available
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