C0013-04J
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
DB ECrossreactivity
HuAccession #
NP_005148Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-c-Abl, phosphorylated (Tyr204) (ABL, Tyrosine-protein Kinase ABL1, Abelson Murine Leukemia Viral Oncogene Homolog 1, Proto-oncogene c-Abl, p150, ABL1, JTK7)
ABL1 is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.
Applications
Suitable for use in ELISA and Dot Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Dot Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr204 of human ABL (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ABL when phosphorylated at Tyr204.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Barnes, K., et al., Oncogene 24(20):3257-3267 (2005). 2.Kim, Y.R., et al., Genes Chromosomes Cancer 43(1):37-44 (2005). 3.Aichberger, K.J., et al., Blood 105(8):3303-3311 (2005). 4.Wei, G., et al., J. Biol. Chem. 280(13):12271-12278 (2005). 5.Robinson, H.M., et al., Leukemia 19(4):564-571 (2005).USBio References
No references available