Technical Data

C0022-10
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
7G10
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-C/EBP beta (CCAAT/Enhancer-Binding Protein beta)

CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPa, C/EBPb, C/EBPd, C/EBPe, and C/EBPg. Among these proteins, C/EBPb is usually highly expressed in liver, intestine, lung and adipose. C/EBPb has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to aa1-271 from human C/EBPb purified from E. coli (NP_005185).
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human C/EBPb. Species Crossreactivity: Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lekstrom-Himes J. et al. (1998) J Biol. Chem. 273, 28545-28548. 2. Parkin SE. et al. (2002) J Biol. Chem.; 277, 23563-23572. 3. Su WC. et al. (2003) J Biol. Chem. 278, 51150-51158
USBio References
No references available
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