c-erbB-2 is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein. The cerbB-2 protein is overexpressed in a variety of carcinomas especially those of breast and ovary.

Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Immunoprecipitation (Native and Denatured) (Use Protein G): 2ug/mg protein lysate Western Blot: 1-2ug/ml for 2hr at RT Immunohistochemistry: 1:200-1:400 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.

SKBR-3 or T47D cells, or breast carcinoma.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein, MW: 185kD. Cellular Localization: Cell membrane.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography.

Recognizes human c-erbB-2 (HER-2, neu). Species Crossreactivity: mouse and rat.