Technical Data

C0026-11NX-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
3F33 (e2–4001)
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-c-erbB2 (HER-2, neu Receptor) (BSA & Azide Free) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000

c-erbB-2 is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein. The cerbB-2 protein is overexpressed in a variety of carcinomas especially those of breast and ovary.
Applications
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunoprecipitation (Native and Denatured) (Use Protein G): 2ug/mg protein lysate Western Blot: 1-2ug/ml for 2hr at RT Immunohistochemistry: 1:200-1:400 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control
SKBR-3 or T47D cells, or breast carcinoma.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein, MW: 185kD. Cellular Localization: Cell membrane.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human c-erbB-2 (HER-2, neu). Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Tandon, A. K., et al. (1989). J Clin Oncol 7:1120–8. 2. Slamon, D. J., et. al. (1989) Science 244:707–12.
USBio References
No references available
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