C0108-34
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
3G42 (HECD-1)Grade
Highly PurifiedApplications
E FC IF IHC IP WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Mouse Anti-Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
The cadherin superfamily comprises over forty proteins which are ~50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Inhibition, Immunoprecipitation, Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000 (immobilized A431 cells) Western Blot: 1-10ug/ml Immunohistochemistry (Frozen or FFPE): ~10ug/ml Inhibition of E-cadherin-dependent cell-cell contact: ~200ug/ml Immunoprecipitation: ~5ug/IP reaction Flow Cytometry: ~10ug/ml Immunofluoresence: ~2-5ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
P3X63-Ag8-U1myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human breast tumor cell line MCF-7.
Form
Supplied as a lyophilized powder from PBS, 1% BSA. No preservatives added. Reconstitute with 50ul sterile ddH2O. Recommended Diluent: 20mM TBS, pH 7.4, 10mM calcium chloride, 1% BSA, 0.09% sodium azide
Purity
Purified by ammonium sulfate precipitation and anion exchange chromatography.
Specificity
Recognizes human epithelial Cadherin E(E-cadherin, uvomorulin).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Marrs J. A., and Nelson W. J. (1996) International Review of Cytology 165:159-205. 2. Takeichi M.(1991) Science 251:1451-1455. 3. Shapiro L., et al and Hendrickson, W. A. (1995) Nature 374: 327-337. 4. Ozawa M, Baribault H. and Kemier R. (1989) EMBO J. 8:1711-1717. 5. Aberle H., et al, and Hoschuetzky H. (1994) J. Cell Sci. 107:3655-3663. 6. Huber A. H., Nelson W. J., and Weis W. I. (1997) Cell 90:871-882. 7. Jiang W. G. (1996) British Journal of Surgery 83:437-446. 8. Schofield K., D’Aquila T and Rimm D. L. (1997) Cancer 81:293-298. 9. Soler A. P., et al, and Keshgegian A. A. (1997) American Journal of Pathology 151:471-478 10. Horiguchi, Y., et al; J.Histochem. Cytochem. 42(10): 1333-1340 (1994). 11. Siitonen, S.M. et al; Am J Clin Pathol 105:394-402 (1996). 12. Rasbridge et al; J. Pathol 169:245-250 (1993). 13. Jankowski, J.A. et al;Internationl J. of Oncology 4(2):441 (1994). 14. Blouvelt, A. et al; The J. of Investigative Dermatology 104:293-296 (1995). 15. Katayama, M.: Int. J. Oncology 5:1049-1057 (1994). 16. Sommers, C. et al; Cancer Res. 54:3544-3552 (1994). 17. Hoover, K.B., et al; Amer. J. Pathol. 153 (6):1767-1773 (1998). 18. Hirano, S. et al: Cell 70:293-301 (1992). 19. Watabe, M.: J Cell Biology 127(1): 247-256 (1994). 20. Shibamoto, S. et al; J Cell Biol. 128:949-957 (1995). 21. Bailey, T. et al; J Pathol 152 (1):135-144 (1998). 22. Braga, V.M., et al; J. Cell Bio. 137(6):1421-1431(1997). 23. Day, M.L., et al; J. Biol. Chem. 274:9656-9664 (1999)USBio References
No references available