C0108-35M-ML650
Clone Type
MonoclonalHost
MouseSource
ChickenConjugate
MaxLight™650Isotype
IgG1,kClone Number
3G49 (3B9)Grade
Affinity PurifiedApplications
IC IF IHC IP WBCrossreactivity
Ch Hu Mo Po RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Cadherin N (Cadherin 2, Neuronal, CDH2) (MaxLight 650)
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins beta-catenin and gamma catenin, which both bind to the actin binding protein alpha catenin.
Applications
Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Formalin fixed, paraffin embedded tissues. Heat induced epitope retrieval required. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to the intracellular domain of chicken N-cadherin.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes chicken N-cadherin. Species Crossreactivity: human, mouse, rat and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Marrs, J.A. and Nelson W.J., International Review of Cytology 165: 159-205 (1996). 2. Takeichi, M., (1991) Science 251: 1451-1455 (1991). 3. Shapiro, L., et al., Nature 374: 327-337 (1995). 4. Ozawa, M., Baribault, H. and Kemier, R., (1989) EMBO J. 8: 1711-1717 (1989). 5. Aberle, H., et al., J. Cell Sci. 107: 3655-3663 (1994). 6. Huber, A.H., Nelson, W.J. and Weis, W.I., Cell 90: 871-882 (1997). 7. Jiang, W.G., British Journal of Surgery 83: 437-446 (1996). 8. Schofield, K., D’Aquila, T. and Rimm, D.L., Cancer 81: 293-298 (1997). 9. Soler, A.P., et al., American Journal of Pathology 151: 471-478 (1997).USBio References
No references available