Goat Anti-Calcitonin Gene Related Peptide (CGRP, Calcitonin Gene-related Peptide 1, CGRP1, Calcitonin Gene-related Peptide I, CGRP I, CGRP-I, Alpha Type CGRP, CAL6, Cal1, Calc, Calcitonin 1, CALC 1, CALC 2, RATCAL6, Calcitonin-related Polypeptide alpha, CALC A, CALCA) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
Applications
Suitable for use in FLISA, Immunofluorescence and Immunohistochemistry. Not recommended for resin sections. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Paraffin and frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to aa23-37 of rat Tyr-CGRP conjugated to gamma globulin.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat Calcitonin gene-related peptide, also known as CGRP. Reacts with both the whole molecule (aa1-37) and the C-terminal fragment (23-37). Species Crossreactivity: mouse, guinea pig and emu.
